Fig. 1

The effect of NC on breast cancer parental cells and mammospheres viability was measured using MTT based. a, b NC had effects on cell proliferation of breast cancer MDA-MB-468 and MCF-7 parental cells and mammospheres in a concentration dependent manner. However, sphere-derived cells showed relative resistance to NC compared to parental cells. c, d Both Parental cells and mammospheres were treated with indicated concentrations of NC. However, no obviously cell death was observed by NC at 1.0 μM for 24, 48 and 72 h in MTT assay. *P < 0.05 compared with the control. The data are presented as the mean ± SD of three separate experiments