Fig. 2
H2O2 induces MMP disruption in HK1 and NP69 cells. HK1 and NP69 cells were treated with H2O2 as described in Fig. 1. The cells were then subjected to flow cytometric analysis of MMP disruption as described in “Methods” section. Percentages of apoptotic cells showing MMP disruption were determined in (a) (i) HK1 and (b) (i) NP69 after treatment with H2O2. Means and SD of two independent experiments performed in duplicate are shown. The differences between untreated control and treated groups were compared by using Student’s t test, *p < 0.05. Representative contour plot diagrams showing the apoptotic populations of (a) (ii) H2O2-treated HK1 cells and (b) (ii) H2O2-treated NP69 cells determined by JC-1 staining are shown. The upper quadrants represent viable cells while the lower quadrants show apoptotic cells