Fig. 2
Exogenous H2S prevented apoptosis of RAECs. a RAECs were stained by TUNEL and Hoechst/PI. The apoptotic cells were stained in red in TUNEL assay. The nuclei of apoptotic cells showed condensate and necrotic cells were stained in red in Hoechst/PI assay. The quantity of positive cells was measured at least 200 cells in three different experiments. (***p < 0.001 vs control group. ##p < 0.01 vs HG + Pal group. ###p < 0.001 vs HG + Pal group). b The expression of cl-casp 3, Bax and Bcl-2 was tested by Western blotting. Each optical density (OD) value was standardized to that of β-actin. There data were summarized from at least three different experiments. (*p < 0.05 vs control group. **p < 0.01 vs control group. ***p < 0.001 vs control group. #p < 0.05 vs HG + Pal group. ##p < 0.01 vs HG + Pal group). c JC-1 measured the mitochondrial membrane potential (ΔΨm). The effect of NaHS treatment on mitochondrial membrane potential in RAECs treated by HG + Pal. JC-1 spontaneously forms J-aggregates and exhibits red fluorescence (590 nm) under a high membrane potential. The JC-1 monomeric form shows green fluorescence (528 nm) under a low potential. The red fluorescence to green fluorescence ratio was measured. (**p < 0.01 vs control group. #p < 0.01 vs HG + Pal group). d The expression of cl-casp 9 was examined by Western blotting. The OD value was standardized to that of β-actin. There data were summarized from at least three different experiments. (**p < 0.01 vs control group. ##p < 0.01 vs HG + Pal group)