Fig. 6

Ammonium salts do not interfere with the core apoptotic machinery. Sp2/0-Ag14 cells were deprived of l-glutamine (Gln) for 3 h in the presence of 5 mM ammonium salt/sodium salt or an equivalent volume of PBS. a Caspase-3 activity assay. b Caspase-3 processing. The arrow and the star indicate the unprocessed, inactive form and the cleaved, active form of caspase-3, respectively. c cleavage of PARP and Lamin A/C. The arrow and the star respectively indicate the full length and the caspase-3 cleaved forms of PARP and Lamin. Ponceau staining of the membrane was used as a loading control. d DNA fragmentation analysis. e cytosolic cytochrome c release. In both panels b and e, actin was used as a gel loading control. SC sodium chloride, SA sodium acetate, AC ammonium chloride. AA ammonium acetate