Fig. 4
Accessibility of VDR and GAPDH proximal promoters in HL60, KG1 and KG1-CtrA. HL60, KG1 and KG1-CtrA cells were treated with nucleases in order to stimulate chromatin digestion after which chromatin was isolated, followed by Real-time PCR. The difference in CT values between digested and undigested chromatin allowed for evaluation of chromatin states (% chromatin accessibility) among the cell lines. GAPDH was used as the control (constitutively expressed) and RHO was used as the reference (epigenetically silenced) gene. Data are the mean values (±SEM) of three individual experiments