Fig. 2

Expression of VDR gene and regulation of VDR and protein in AML cell lines in response to 1,25D and ATRA. KG1, HL60, KG1-CtrA and KG1-RARA cells were exposed to 10 nM 1,25D or to 500 nM ATRA or both for 72 h and then expression of VDR protein was studied (a). The cells were fractionated into cytosolic, nucleosolic and chromatin-bound fractions. Fractionated proteins were analyzed in western blots using anti-VDR, anti-actin and anti-Histone antibodies. The experiments were repeated from 2 (HL60) to 5 (KG1) times and representative blots are presented. C control, D 10 nM 1,25D; A 500 nM ATRA. The constitutive expression levels of VDR mRNA (b) in the above cell lines were measured by Real-time PCR relative to GAPDH expression levels. The bar charts show mean values of three experiments (±SEM) of relative quantity (RQ)