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Table 1 Activity of respiratory complex III and of citrate synthase in UMSBP deleted isolated amastigotes for the indicated time point

From: Universal minicircle sequence binding protein of Leishmania donovani regulates pathogenicity by controlling expression of cytochrome-b

Specific activity of respiratory complex-III and of citrate synthase in amastigotes isolated from macrophages
Enzymes Control (WT) WT + ANTIMYCINA (72 h) LdU−/+ LdU−/+ AB
24 h 48 h 72 h 72 h
ComplexIII activity/min/mg protein 110.4 ± 9.3 26.4 ± 1.8 45.3 ± 3.2 19.4 ± 1.9 8.2 ± 0.8 104 ± 9.1
Citrate synthase activity 492.5 ± 26.2 452 ± 23.1 416 ± 19.8 46 ± 2.8 478.2 ± 24.2
  1. Antimycin A was used as positive control to specifically inhibit complex III. Activity of complex III was reduced significantly compared to the control (WT) in response to LdUMSBP deletion. After episomal expression of WT LdUMSBP in LdU−/+ parasites, the activity of complex-III of isolated mitochondria from amastigotes was restored near to WT activity in LdU−/+ AB parasites. Citrate synthase was used to monitor the intactness of mitochondria. Up to 48 h of infection, mitochondria was intact and at 72 h mitochondria was disrupted. In add back amastigotes mitochondria were found to be intact
  2. Rates are mean ± SEM