Fig. 3

Reconstituted LdTXN–LdTXNPx reaction regulates the binding of LdUMSBP to UMS DNA. rLdUMSBP was incubated with TXN, as indicated, in a TXN reaction mixture (A, Panel A) and the reaction was followed by the addition with the indicated concentrations of TXNPx (B, Panel A) as described under “Methods”, followed by the UMS-binding assay (EMSA). A, Panel B and B, Panel B Phosphorimaging quantification of EMSA data. The percentage of complex formation presented relative to the value measured in the absence of TXNPx in a reaction mixture supplemented with 0.9 mM TXN was used as a 100 % (B Panel A). Lane-a of (A) and (B) correspond to no protein