Fig. 4

Effect of CLIC4 on ATP-induced mitochondrial membrane depolarization. Traces (a) and summarized data (b) showing 100 μmol/L ATP-induced changes in the HN4 cell mitochondrial membrane potential, which was detected by the cell-permeable membrane potential sensitive-fluorescent indicator rhodamine-123. The change in the membrane potential was indicated by the ratio of the fluorescence density before and after the application of ATP. The ratio increase represents the membrane potential depolarization. The HN4 cells were transfected with CLIC4 (CLIC4 siRNA) or scrambled (Con) siRNA. Values are shown as the mean ± SE. n = 5. *P < 0.05. vs. control (Con) group