Fig. 2

The binding between full-length TLS and the fragment 1–1. a Computational analysis predicting the secondary structure of pncRNA-D by CentroidFold (http://www.ncrna.org/centroidfold/). The position of the fragment 1–1 is shown in red box. b Imino-imino (left) and imino-amino/base (right) proton regions of a NOESY spectrum with a mixing time of 300 ms, the assignments of imino protons being indicated. Cross peaks to two amino protons and H2 are boxed, respectively. c 1D imino proton spectra of the fragment 1–1, the 3′ end of the fragment 1–1, G49A mutant of the 3′ end of the fragment 1–1 and G46A mutant of the 3′ end of the fragment 1–1, respectively, from top to bottom. d The two possible secondary structures of the fragment 1–1, the upper one being concluded as a right one. e Western blot analysis were performed to detect the binding between 5′ and 3′ ends of the fragment 1–1. Ten percent of the protein used for RNA pull down assay was loaded as input N = 5