Fig. 6

Knockdown of hMYH affects chromatin association of hTopBP1. siGFP or siMYH transfected HEK293 cells were either untreated or treated with 20 mM HU for 1 h. The cells were lysed and chromatins were collected. Chromatin binding proteins were analyzed by western blotting. ORC2 were used loading control. Quantification of the association of hTopBP1 with chromatin is shown as mean ± standard error (of three independent experiments). P values were calculated using a paired t test, **p < 0.01