Fig. 3

Identification of the hMYH-interacting domain of hTopBP1. a Schematic map of the hTopBP1 deletion mutants to identify the interacting region of hTopBP1. b Co-IP and immunoblotting were performed on lysates expressing the various hTopBP1 deletion mutants. For the experiments, HEK293 cells were co-transfected with full-length c-Myc-hMYH and the various GST-hTopBP1 mutants. Co-IP was performed with an anti-c-Myc antibody, and the precipitated proteins were immunoblotted with anti-GST and anti-c-Myc antibodies