Fig. 6
Effect of ferulic acid (FA) and aspirin (ASP) chemopreventive regimen on apoptotic proteins. Immunohistochemical analysis was performed on paraffin-embedded and micro-sectioned pancreatic tissues as described in methods section. a–c Representative images showing effect of modified FA and ASP c-SLN combination on p-RB, p21, and p-ERK1/2 protein expressions in pancreatic tissue, respectively. d–f An increase in the p-RB, p21 and p-ERK1/2 protein expressions was observed in FA + ASP c-SLN treated groups compared to the vehicle control group. All the pictures were taken at ×200 magnification. Statistical significance was determined by one-way ANOVA followed by Dunnett’s multiple comparison test post hoc analysis. ***p < 0.001 represents statistical significance between blank c-SLN vehicle control and FA + ASP c-SLN treatment