Application of CRISPR/Cas9 for biomedical discoveries

Cell & Bioscience

Table 1 Major components of each CRISPR type^a

Action	Type I	Type II	Type III
Spacer acquisition	Cas1/Cas2	Cas1/Cas2/Csn1/Cas9	Cas1/Cas2
Pre-cRNAprocessing	Cas6 or Cas5	Rnase III	Cas6
Interference Complex members	Cascade	tracrRNA/Cas9	Csm/Cmr
Secondary processing	None	5′ end cleavage	3′ end cleavage
Interference Subtubes	I-A, I-B, I-E, I-F, I-C,	II-A, II-B, II-C	III-A, III-B

^aCRISPR types are characterized by their operon composition the involved Cas proteins and the structure of the repeats in the CRISPR array. This table presents the defining characteristics of each type [27, 28]. Cascade = CRISPR-associated complex for antiviral defense

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