Fig. 3From: Hypoxia induces calpain activity and degrades SMAD2 to attenuate TGFβ signaling in macrophagesSMAD2 protein stability is reduced in response to TGFß/hypoxia. a mRNA expression of SMAD2 followed by quantitative PCR in macrophages stimulated for 8 h with TGFß under normoxia (nor) vs. hypoxia (hy). b Macrophages were stimulated with TGFß for 4 h, followed by the addition of cycloheximide for 0 h to 4 h and subsequent Western blot analysis of SMAD2 expression. c Half-life determination of SMAD2 in TGFß-stimulated macrophages under normoxia (nor) vs. hypoxia (hy) as a result of experiments performed in Fig. 3b Back to article page