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Fig. 1 | Cell & Bioscience

Fig. 1

From: Transient blocking of NK cell function with small molecule inhibitors for helper dependant adenoviral vector-mediated gene delivery

Fig. 1

Ruxolitinib and CAPE block HD-Ad gene therapy mediated activation of macrophages. THP-1 cells were activated by 5 ng/ml PMA to prepare mature macrophages. Mature macrophages were harvested and cultured in presence and absence of ruxolitinib or CAPE, and in presence of both the inhibitors. As depicted in figure one cohort of these culture conditions were transduced with HD-Ad and other cohort was not transduced. After culturing them for 24 h total RNA was isolated and analysed by qRT- PCR to look for relative expression of different cytokines. Relative expression of cytokines from one experiment is depicted in the figure. All the data is normalised with GAPDH expression. The significance was calculated by one-way ANOVA with Tukey’s multiple comparison. *p < 0.05, **p < 0.01, ***p < 0.001

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