Figure 5

Point mutations in the selective filter region reduced the inhibitory effect of LrB on mKv1.3. (A) Representative mKv1.3 current traces at +50 mV in the absence (Ctl) or presence of 3 or 10 μM LrB. (B) Concentration-response curves of LrB inhibition on currents mediated by the wild-type or mKv1.3 mutants taken at +50 mV. Each data point represents mean ± SE of at least five experiments. (C) Representative mKv1.3 current traces at +50 mV in the absence (Ctl) or presence of 10 or 100 pM ADWX-1. (D) Concentration-response curves of ADWX-1 inhibition on currents mediated by the wild-type or mKv1.3 mutants taken at + 50 mV. Each data point represents mean ± SE of at least five experiments.