Figure 1

ESE-1 and GP73 expressions were induced by IL-1β stimulation in vitro . (A) The basal levels of ESE-1 and GP73 expressions in HepG2, Hep3B, and Huh7 cells were analyzed by conducting Western blot. (B) Hep3B cells were stimulated with different doses of IL-1β (2.5, 10, and 20 ng/mL) for 48 h. IL-1β-induced ESE-1 and GP73 mRNA and protein levels were analyzed by performing qPCR and Western blot. (C) Huh7 cells were stimulated with different doses of IL-1β (10 and 20 ng/mL) for 48 h. ESE-1 and GP73 mRNA and protein levels were analyzed with qPCR and Western blot. The values were normalized to GAPDH. Western blot was reprobed for GAPDH as a loading reference control.