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Figure 3 | Cell & Bioscience

Figure 3

From: Modulation of the stability and activities of HIV-1 Tat by its ubiquitination and carboxyl-terminal region

Figure 3

Tat transactivation activity is modulated by its ubiquitination and carboxyl-terminal region. (A) 293 T cells were cotransfected with an HIV-1 LTR-driven luciferase plasmid, a GFP-Tat101 plasmid, and various ubiquitin mutants and treated with or without MG132. Luciferase activity was then measured and normalized to GFP intensity. Bars represent the relative folds of transactivation activity obtained from two experiments done in triplicate and normalized against the untreated ubiquitin-K0 mutant transfection group. Results were expressed as the mean ± SD. (B) 293 T cells were cotransfected with an HIV-1 LTR-driven luciferase plasmid and plasmids expressing GFP fusion proteins or GFP alone and treated with or without MG132. The relative folds of transactivation activity were obtained as in (A). Results were expressed as the mean ± SD. Two-tailed Student’s t-test for all graphs. *P < 0.05, **P <0.01, ***P < 0.001; ns, not significant.

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