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Figure 7 | Cell & Bioscience

Figure 7

From: Absence of Appl2 sensitizes endotoxin shock through activation of PI3K/Akt pathway

Figure 7

Appl1 KO mice show less severe endotoxic shock than WT mice. (A) Diagram showing the construction of Appl1 KO mouse. (B) Western blot analysis demonstrating the absence of the Appl1 protein in the macrophages of Appl1KO mice. (C) Kaplan-Meier survival curves of the WT (n = 9) and the Appl1 KO (n = 9) mice after LPS challenge (30 mg/kg weight, intraperitoneal injection). Compared with the WT mice, the Appl1 KO mice were less susceptible to the endotoxin challenge. (D) Photographs of the WT and Appl1 KO mice 24 h after intraperitoneal injection of LPS. Appl1 KO mice showed only mild effects, whereas WT mice exhibited typical signs of endotoxemia. (E) HE-stained kidney sections from mice at 24 h post-LPS challenge. (F) Mouse serum TNFα level at 60 min after LPS injection. (G) Kinetics of the IL-1β expression in the LPS-stimulated Appl1 KO and control mice. Scale bar: 50 μm; Appl1−/−, Appl1 KO mouse; *p < 0.05, compared with WT mice, n = 6.

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