Figure 6From: Absence of Appl2 sensitizes endotoxin shock through activation of PI3K/Akt pathwayThe p85α subunit of PI3K associates with Appl 1 and 2. Peritoneal macrophages from WT mice were treated with 1 μg/mL LPS for 20 min. Macrophages lysates were immunoprecipitated with IgG, Appl2 and Appl1 antibody and immuoblotted using antibodies against TLR4, MyD88, Akt, p85α, Appl1 and Appl2, respectively.Back to article page