Genome sequencing accuracy by RCA-seq versus long PCR template cloning and sequencing in identification of human papillomavirus type 58

Table 3 Nucleotide substitutions by long-PCR amplification in HPV58 isolates create or inactivate restriction enzyme digestion sites

Summarized in this table are distinguishable restriction enzyme cutting sites due to nucleotide substitutions introduced by long PCR amplification in three HPV58 isolates. See other details from Figure 3 legends. *The numbers are nucleotide positions with the substitutions to the reference HPV58 genome.

ISSN: 2045-3701