Genome sequencing accuracy by RCA-seq versus long PCR template cloning and sequencing in identification of human papillomavirus type 58

Table 2 Nucleotide insertion and deletion created by long-PCR amplification in HPV58 isolates

Summarized in this table are nucleotide insertions/deletions introduced by long PCR amplification from the two individual clones in each of three HPV58 isolates. S1 and S2 denotes Sanger sequence #1 (clone #1) and #2 (clone #2), respectively. Numbers are nucleotide positions in the reference HPV58 genome at where the insertion or deletion was identified in each HPV58 isolate.

ISSN: 2045-3701