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Figure 1 | Cell & Bioscience

Figure 1

From: Comparative analysis of the activation of unfolded protein response by spike proteins of severe acute respiratory syndrome coronavirus and human coronavirus HKU1

Figure 1

Expression and localization of SARS-CoV and HCoV-HKU1 S proteins. (A) Western blot analysis. 293FT cells were transfected with pLenti-SCV-S and pLenti-HKU1-S constructs expressing V5-tagged SARS-CoV S protein (SCV-S) and HCoV-HKU1 S protein (HKU1-S), respectively. Cells in the control group received empty p-Lenti vector only. Cells were lysed and immunoblotted with anti-V5 and anti-α-tubulin antibodies. (B) Confocal immunostaining. SARS-CoV and HCoV-HKU1 S proteins were expressed in HeLa cells and stained with anti-V5 antibody (panels 1 and 4). DsRed-ER was used as an ER marker (panels 2 and 5). The S (green) and DsRed-ER (red) fluorescent signals are overlaid and colocalization is in yellow (panels 3 and 6). Nuclear morphology was visualized with DAPI and is in blue. Bar, 20 μM.

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Cell & Bioscience

ISSN: 2045-3701

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