Generation of gene disruptions by transcription activator-like effector nucleases (TALENs) in Xenopus tropicalis embryos

Table 8 PCR mix for amplification of target genomic DNA

Components	PCR Mix (μL)
5 × GoTaq Buffer	5
25 mM MgCl₂	2.5
10 mM dNTP Mix (Fermentas)	0.5
Primer 1/2 (10 mM)	0.5
Primer 3 (10 mM)	0.5
Genomic DNA	0.5
GoTaq DNA polymerase (Promega)	0.5
dH₂O	15
Total	25

ISSN: 2045-3701