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Table 6 Ligation program in Golden Gate cloning round 2

From: Generation of gene disruptions by transcription activator-like effector nucleases (TALENs) in Xenopus tropicalis embryos

Cycle number

Temperature (°C)

Time (min)

1-3

16

10

 

37

20

4

16

20

5

55

20

6

80

5