Generation of gene disruptions by transcription activator-like effector nucleases (TALENs) in Xenopus tropicalis embryos

Table 5 Ligation mix in Golden Gate cloning round 2

Components	pCS2-TALEN Mix (μL)
Buffer 3 (NEB)	1
pFUS_A-RVDs vectors (150 ng/μL)	0.8
pFUS_B-RVDs vectors (150 ng/μL)	0.8
Last Repeats vectors (150 ng/μL)	0.6
BsmbI (NEB)	0.8
pCS2-TALEN-ELD/KKR vectors (150 ng/μL)	1
dH₂O	5
Total	10

ISSN: 2045-3701