Generation of gene disruptions by transcription activator-like effector nucleases (TALENs) in Xenopus tropicalis embryos

Table 3 Colony PCR mix in Golden Gate cloning round 1

Components	PCR Mix (μL)
5 × GoTaq Buffer	5
25 mM MgCl₂	2.5
10 mM dNTP Mix (Fermentas)	0.5
Primer Fw (10 mM)	0.5
Primer Re (10 mM)	0.5
Colony suspension	5
GoTaq DNA polymerase (Promega)	0.5
dH₂O	10.5
Total	25

ISSN: 2045-3701