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Table 2 Ligation program in Golden Gate cloning round 1

From: Generation of gene disruptions by transcription activator-like effector nucleases (TALENs) in Xenopus tropicalis embryos

Cycle number

Temperature (°C)

Time (min)

1-15

37

5

 

16

10

16

16

15

17

50

10

18

80

5

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Cell & Bioscience

ISSN: 2045-3701

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