Table 1 Ligation mix in Golden Gate cloning round 1
Components | pFUS_A Mix (μL) | pFUS_B Mix (μL) |
|---|---|---|
Buffer 4 (NEB) | 1 | 1 |
T4 Ligase (NEB) | 0.8 | 0.8 |
BsaI (NEB) | 0.8 | 0.8 |
RVD vectors (150 ng/μL) | 0.6 × 10 | 0.6 × #N-1 |
pFUS_A/B vectors (150 ng/μL) | 1 | 1 |
ATP (25Â mM) | 0.4 | 0.4 |
dH2O | 0 | 6-(0.6 × #N-1) |
Total | 10 | 10 |