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Figure 3 | Cell & Bioscience

Figure 3

From: Anti-oxidative stress regulator NF-E2-related factor 2 mediates the adaptive induction of antioxidant and detoxifying enzymes by lipid peroxidation metabolite 4-hydroxynonenal

Figure 3

Effects of NRF2 on the mRNA expression of metabolizing and antioxidant enzymes. (A) HeLa cells were treated with 0, 0.1, 1 and 10 μM of 4-HNE for 6 h. mRNA expression of AKR1C1, GSTA4 and HO-1 was induced by 4-HNE in a dose-dependent manner. (B) HeLa cells were transfected with NRF2 specific or non-specific siRNA for 24 h, followed by 4-HNE treatment for 6 h. Knocking-down of NRF2 attenuated the induction of AKR1C1, GSTA4 and HO- 1by 4-HNE. Densitometry analyses of the PCR products were performed by Image J program, and the numbers at the top of each band are relative density compared to the control.

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Cell & Bioscience

ISSN: 2045-3701

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