Figure 2

MHC class I H-2^bbinding restriction of LT aa 19–27 epitope identified by intracellular cytokine staining and flow cytometry. MHC class I binding restriction was determined by C1R transfectants, C1R/D^b and C1R/K^b , that were pulsed with immunodominant LT peptide (aa 19–27). (A) Representative flow cytometric data showing the amounts of LT-specific IFN-γ⁺ CD8⁺ T-cells after splenocytes from pcDNA3-CRT/LT vaccinated mice are stimulated by LT peptide-pulsed C1R, C1R/D^b, or C1R/K^b cells in vitro. (B) Representative bar graph of flow cytometric data showing the proportions of LT-specific IFN-γ + CD8+ T cells per 3 × 10⁵ splenocytes following in vitro stimulation as described above. Note that LT epitope (aa 19–27) is H-2k^b-restricted.