Figure 1

Time course characteristics of protein relocalization elicited by hypoxia or reoxygenation. (A) The time courses of relocalization of Snf11 and Swp82 in response to hypoxia. Cells expressing Snf11-GFP or Swp82-GFP were grown in air and then shifted to hypoxic growth conditions. At various time points, cells were imaged, and the number of cells showing GFP-tagged proteins in the nucleus (N) or cytosol (C) was counted. The percentage of cells showing nuclear locations is calculated and plotted. (B) The time courses of relocalization of Snf5 and Swi3 in response to reoxygenation. Cells expressing Snf5-GFP or Swi3-GFP were grown under hypoxia and then shifted to normoxic growth conditions. At various time points, cells were imaged, and the number of cells showing GFP-tagged proteins in the nucleus (N) or cytosol (C) was counted. The percentage of cells showing nuclear localization is plotted here.