Figure 3

Characterization of PAT-9 expression. Localization of the PAT-9 protein as determined by use of a PAT-9::GFP translational fusion in the transgenic rescued pat-9(st558) stain. Fluorescence microscopy shows the localization of PAT-9::GFP within muscle nuclei in a A) 2-fold embryo, B) larva and C) adult animal. The anti-PAT-9 antibody stains body wall muscle nuclei of wild-type embryos (D), as well as the nuclei of young larvae (E) and adults (F); PAT-9 immunostaining shows that PAT-9 is expressed in germ-like nuclei in cytoplasmic syncytium of the C. elegans gonad (G). Endogenous PAT-9 (G and J) is nuclear and localized to the DAPI-poor region as shown by (H and K) DAPI staining, and (I and L) merging with PAT-9 immunostaining. Bars =10 μm.