Figure 1

Lithium induced cell number increase in neural precursor cultures. (A) Lithium chloride dose dependently stimulated cell number increase in RG3.6 cell cultures. Cell count analysis was performed on cultures of RG3.6 cells treated with the indicated doses of LiCl for 7 days. *: Bonferroni/Dunn, P < 0.002 compared to no LiCl control. (B) Lithium chloride but not sodium chloride induced cell number increase in RG3.6 cell cultures. Cell count analysis was performed on cultures of RG3.6 cells treated with control medium, 3 mM NaCl or 3 mM LiCl for 5 days. *: paired t test, P < 0.01 compared to 3 mM NaCl or control medium treatment. (C) Lithium stimulated cell number increase in rat primary neural precursor cell cultures. Cell count analysis was performed on cultures of rat primary neural precursor cells treated with 3 mM NaCl or 3 mM LiCl for 5 days. *: Bonferroni/Dunn, P < 0.002. (D) Lithium stimulated cell number increase in mouse primary neural precursor cell cultures. Cell count analysis was performed on cultures of mouse primary neural precursor cells treated with 3 mM NaCl or 3 mM LiCl for 5 days. *: Bonferroni/Dunn, P < 0.002. The data represent mean ± standard error (n = 6 for each condition).